| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPP30 |
| Uniprot No | P78346 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-268aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMAVFADLDLRAGSDLKALRGLVETAAH LGYSVVAINHIVDFKEKKQEIEKPVAVSELFTTLPIVQGKSRPIKILTRL TIIVSDPSHCNVLRATSSRARLYDVVAVFPKTEKLFHIACTHLDVDLVCI TVTEKLPFYFKRPPINVAIDRGLAFELVYSPAIKDSTMRRYTISSALNLM QICKGKNVIISSAAERPLEIRGPYDVANLGLLFGLSESDAKAAVSTNCRA ALLHGETRKTAFGIISTVKKPRPSEGDEDCLPASKKAKCEG |
| 预测分子量 | 32 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RPP30重组蛋白的参考文献示例(注：部分内容为模拟示例，实际文献需通过学术数据库核实)：
1. **标题**：*Expression and Functional Characterization of Recombinant Human RPP30 in Escherichia coli*
**作者**：Chen L, et al.
**摘要**：本研究成功在大肠杆菌中表达并纯化了重组人源RPP30蛋白，通过体外酶活实验证实其参与核糖核酸酶P的催化功能，为研究RNA加工机制提供了实验基础。
2. **标题**：*Development of a RPP30-Based Fluorescent Probe for Pathogen Detection*
**作者**：Wang T, et al.
**摘要**：利用重组RPP30蛋白设计了一种新型荧光探针，通过特异性结合病原体RNA实现快速检测，在临床样本中展现出高灵敏度和低交叉反应性。
3. **标题**：*Crystal Structure of Recombinant RPP30 Reveals Insights into RNA Binding*
**作者**：Johnson R, et al.
**摘要**：通过X射线晶体学解析了重组RPP30的原子级结构，揭示了其与tRNA前体结合的活性位点，为靶向RNA加工酶的药物开发提供结构依据。
4. **标题**：*RPP30 Recombinant Protein as a Quality Control Marker in Gene Therapy*
**作者**：Martinez S, et al.
**摘要**：将重组RPP30蛋白作为宿主细胞DNA残留检测的内参标准品，优化了基因治疗产品的质量控制流程，提升了检测的准确性和可重复性。
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**建议**：如需真实文献，可通过PubMed或Google Scholar搜索关键词“RPP30 recombinant protein”、“RPP30 expression and purification”或结合具体应用方向(如“diagnostics”、“CRISPR”)进行检索。
Recombinant RPP30 protein is a human-derived protein widely utilized in molecular biology and diagnostic applications. RPP30 (Ribonuclease P Protein Subunit p30) is a critical component of the RNase P complex, an evolutionarily conserved endonuclease responsible for processing precursor tRNA molecules by cleaving their 5' leader sequences. Unlike the catalytic RNA subunit of RNase P, RPP30 is a protein subunit essential for structural stability and enzymatic activity. The gene encoding RPP30. *RPP30*, is located on chromosome 10 in humans and produces a 30 kDa protein involved in RNA-protein interactions.
In recombinant form, RPP30 is typically expressed in bacterial or eukaryotic systems (e.g., *E. coli* or mammalian cells) using genetic engineering techniques, followed by purification via affinity tags like His-tags. This recombinant protein retains its ability to bind RNA and participate in RNase P-like enzymatic activities, making it valuable for functional studies of ribonucleoprotein complexes. Additionally, recombinant RPP30 serves as a key control in diagnostic assays, particularly CRISPR-based nucleic acid detection platforms (e.g., SHERLOCK or DETECTR). Here, it acts as an endogenous internal control to verify sample integrity and assay functionality, ensuring reliable detection of pathogens like SARS-CoV-2 by confirming the presence of human genetic material in test samples.
Beyond diagnostics, recombinant RPP30 is employed in research exploring RNase P’s role in cellular processes, including tRNA maturation, gene expression regulation, and disease mechanisms. Its recombinant availability enables standardized, high-purity preparations for reproducible experiments, addressing challenges associated with isolating native RNase P complexes. Overall, recombinant RPP30 bridges basic research and clinical applications, underscoring its dual utility in advancing molecular science and precision diagnostics.
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