首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠胃泌素释放肽(GRP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GRP含量。GRP即胃泌素释放肽，是一种神经递质，在多种生理过程中发挥作用。其与细胞生长、增殖相关，在肿瘤发生发展里有重要地位。科研聚焦于研究GRP及其受体的作用机制，以期通过阻断信号通路来实现肿瘤等疾病的诊断与治疗。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，可为神经内分泌研究、消化道生理调控实验以及肿瘤微环境分析（如小细胞肺癌模型中GRP的高表达研究）提供可靠数据支持，配套提供标准品、检测抗体及优化缓冲体系，适用于细胞实验、动物模型或离体组织样本的科研级定量检测需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：GrpGastrin-releasing peptide ELISA Kit; GRP) [Cleaved into: Neuromedin-C ELISA Kit; GRP-10)] ELISA Kit
缩写：GRP
Uniprot No.：P24393
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat GRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 91 | |||||
| Range % | 86-95 | ||||||
| 1:2 | Average % | 102 | |||||
| Range % | 97-107 | ||||||
| 1:4 | Average % | 91 | |||||
| Range % | 85-97 | ||||||
| 1:8 | Average % | 97 | |||||
| Range % | 91-103 | ||||||
回收率：
| The recovery of rat GRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 89-98 | |||||
| EDTA plasma (n=4) | 92 | 88-96 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected10002.6542.5262.5902.4045002.1472.0482.0981.9122501.4461.5621.5041.3181250.9820.9960.9890.80362.50.5680.5510.5600.37431.20.3720.3610.3670.18115.60.2780.2740.2760.09000.1860.1850.186 | |||||||
货期：3-5 working days
×
