首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人胃泌素释放多肽(GRP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、组织培养上清液、组织匀浆样本中的GRP含量。GRP（胃泌素释放肽）是一种胃肠激素，广泛分布于哺乳动物的神经系统、胃肠道和呼吸道。其研究机制主要涉及GRP-R（胃泌素释放肽受体）的拮抗剂，通过调节胃酸分泌、胃肠道运动以及某些癌细胞生长等方面发挥作用。抑制GRP-R可间接调节GRP活性，为研究GRP在生理和病理过程中的作用提供了重要工具。试剂盒检测范围为1.25 ng/mL-80 ng/mL，该产品适用于胃酸分泌调控机制研究、肿瘤微环境中神经肽功能分析，以及神经内分泌系统相关疾病的分子机制探索等科研场景，为体外实验提供可靠的检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：BN ELISA Kit; Bombesin ELISA Kit; GRP ELISA Kit; GRP-10 ELISA Kit; GRP_HUMAN ELISA Kit; GRP10 ELISA Kit; Neuromedin-C ELISA Kit; NeuromedinC ELISA Kit; Pre progastrin releasing peptide ELISA Kit; PreproGRP ELISA Kit; ProGRP ELISA Kit
缩写：GRP
Uniprot No.：P07492
种属：Homo sapiens (Human)
样本类型：serum, cell culture supernates, tissue homogenates
检测范围：1.25 ng/mL-80 ng/mL
灵敏度：0.312 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human GRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 92-101 | |||||
| 1:2 | Average % | 103 | ||||
| Range % | 98-110 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 88-96 | |||||
| 1:8 | Average % | 86 | ||||
| Range % | 80-92 | |||||
回收率：
| The recovery of human GRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 89-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected802.6942.5722.6332.512402.0822.1052.0941.973201.4181.4761.4471.326100.8910.8350.8630.74250.5400.5720.5560.4352.50.3500.3410.3460.2251.250.2760.2620.2690.14800.1240.1170.121 | ||||||
货期：3-5 working days
×
