| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | B5R; DIA1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human CYB5R3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是3篇与CYB5R3抗体相关的文献摘要信息：
---
1. **文献名称**：*"NADH-cytochrome b5 reductase 3 (CYB5R3) promotes tumor growth and metastasis in breast cancer by regulating mitochondrial iron metabolism"*
**作者**：Chen et al. (2021)
**摘要**：研究利用CYB5R3特异性抗体，发现其在乳腺癌中通过调节线粒体铁代谢促进肿瘤生长和转移，高表达CYB5R3与患者预后不良相关。
2. **文献名称**：*"Immunohistochemical analysis of CYB5R3 in human colorectal cancer: association with oxidative stress and chemoresistance"*
**作者**：Sato et al. (2018)
**摘要**：通过CYB5R3抗体的免疫组化分析，发现其在结直肠癌中高表达，与氧化应激增强和化疗耐药性相关，提示其作为治疗靶点的潜力。
3. **文献名称**：*"Functional characterization of CYB5R3 mutations in recessive congenital methemoglobinemia"*
**作者**：Shirabe et al. (1995)
**摘要**：利用CYB5R3抗体进行Western blot和酶活性检测，揭示遗传性高铁血红蛋白血症患者的CYB5R3基因突变导致酶功能缺陷的分子机制。
---
以上文献均涉及CYB5R3抗体在疾病机制或功能研究中的应用，涵盖癌症、代谢紊乱等领域。如需具体DOI或期刊信息可进一步补充。
The CYB5R3 antibody targets cytochrome b5 reductase 3 (CYB5R3), a critical enzyme involved in electron transfer reactions. CYB5R3 exists in two isoforms: a soluble form in erythrocytes and a membrane-bound form in mitochondria and endoplasmic reticulum. It plays a key role in reducing ferric hemoglobin (methemoglobin) to functional ferrous hemoglobin, maintaining oxygen-carrying capacity. Additionally, it participates in fatty acid desaturation, cholesterol synthesis, and drug metabolism by interacting with cytochrome b5.
Mutations in the CYB5R3 gene are linked to recessive congenital methemoglobinemia (Types I and II), characterized by cyanosis and neurological complications. Type II, involving systemic enzyme deficiency, is particularly severe. CYB5R3 dysregulation has also been implicated in cancer, neurodegenerative diseases, and metabolic disorders.
CYB5R3 antibodies are essential tools for studying enzyme expression, localization, and function. They enable detection via Western blotting, immunohistochemistry, and flow cytometry, aiding in research on oxygen metabolism, redox biology, and disease mechanisms. Commercial antibodies are typically validated for specificity against human, mouse, or rat CYB5R3 isoforms. Recent studies also explore its role as a potential biomarker or therapeutic target in conditions like cancer and Alzheimer’s disease.
×
