首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠胃泌素酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GAST含量。小鼠胃泌素是一种由胃窦及小肠黏膜的G细胞分泌的激素，对胃酸分泌和胃肠道黏膜生长有促进作用。研究发现，胃泌素过量表达与多种肿瘤的发生发展密切相关，其作用机制主要通过CCK受体介导，影响细胞增殖、迁移和凋亡等过程。试剂盒检测范围为1.25 pg/mL-80 pg/mL，适用于基础医学研究中探索胃肠道疾病模型、药物干预对胃泌素分泌的影响，或评估胃肠功能调节机制等领域本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Gast ELISA Kit; Gas ELISA Kit; Gastrin [Cleaved into: Gastrin-71 ELISA Kit; G71); Big gastrin ELISA Kit; Gastrin-34 ELISA Kit; G34); Gastrin] ELISA Kit
缩写：GAST
Uniprot No.：P48757
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：1.25 pg/mL-80 pg/mL
灵敏度：0.31 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse Gastrin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 86-99 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 92-105 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 88-97 | |||||
| 1:8 | Average % | 88 | ||||
| Range % | 83-95 | |||||
回收率：
| The recovery of mouse Gastrin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 87-99 | ||||
| EDTA plasma (n=4) | 98 | 95-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected802.7852.6502.7182.555402.3842.3752.3802.217201.6891.7521.7211.558100.9741.0301.0020.83950.5750.5320.5540.3912.50.3620.3570.3600.1971.250.2470.2580.2530.09000.1610.1650.163 | ||||||
货期：3-5 working days
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