首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠谷氨酸脱羧酶1(GAD1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的GAD1含量。GAD1编码谷氨酸脱羧酶，它能催化谷氨酸转化为γ-氨基丁酸（GABA）。在神经系统中，GABA作为重要的抑制性神经递质发挥作用。研究聚焦其在神经调节机制里的功能，探究它与癫痫、精神分裂症等神经精神疾病发病机制的联系，为相关疾病治疗提供方向。试剂盒检测范围为6.25 pg/mL-400 pg/mL，适用于神经生物学研究、疾病动物模型分析以及药物干预实验中GAD1表达水平的动态监测。科研人员可通过定量检测小鼠中枢神经系统、胰岛组织或其他相关样本中GAD1的蛋白浓度变化，为探索GABA能神经元功能、自身免疫性疾病机制及神经系统相关病理过程提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Gad1 ELISA Kit; Gad67Glutamate decarboxylase 1 ELISA Kit; EC 4.1.1.15 ELISA Kit; 67 kDa glutamic acid decarboxylase ELISA Kit; GAD-67 ELISA Kit; Glutamate decarboxylase 67 kDa isoform ELISA Kit
缩写：GAD1
Uniprot No.：P48318
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：6.25 pg/mL-400 pg/mL
灵敏度：1.56 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse GAD1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 102 | ||||
| Range % | 99-105 | |||||
| 1:10 | Average % | 95 | ||||
| Range % | 91-98 | |||||
| 1:20 | Average % | 88 | ||||
| Range % | 84-93 | |||||
| 1:40 | Average % | 99 | ||||
| Range % | 95-104 | |||||
回收率：
| The recovery of mouseGAD1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 104 | 100-109 | ||||
| EDTA plasma (n=4) | 93 | 89-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected4002.9512.8962.9242.7532002.1241.9602.0421.8711001.3391.4111.3751.204500.8420.9020.8720.701250.5990.6420.6210.45012.50.3540.3700.3620.1916.250.2560.2480.2520.08100.1750.1660.171 | ||||||
货期：3-5 working days
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