首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人谷氨酸脱羧酶(GAD)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GAD1含量。GAD1 是重要靶点。它编码谷氨酸脱羧酶，参与神经递质γ-氨基丁酸（GABA）的合成。研究围绕其调控机制展开，探究其在神经发育、神经疾病如癫痫等中的作用，旨在通过调节 GAD1 功能为相关疾病治疗提供新策略。试剂盒检测范围为0.312 ng/mL-20 ng/mL，本试剂盒适用于神经递质代谢研究、糖尿病发病机制探索、药物筛选等科研领域本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：67 kDa glutamic acid decarboxylase ELISA Kit; CPSQ1 ELISA Kit; DCE1 ELISA Kit; DCE1_HUMAN ELISA Kit; EC 4.1.1.15 ELISA Kit; FLJ45882 ELISA Kit; GAD 1 ELISA Kit; GAD 67 ELISA Kit; GAD ELISA Kit; GAD-67 ELISA Kit; GAD1 ELISA Kit; Glutamate decarboxylase 1 (brain; 67kDa) ELISA Kit; Glutamate decarboxylase 1 ELISA Kit; Glutamate decarboxylase 1 brain 67kD ELISA Kit; Glutamate decarboxylase 1 brain 67kDa ELISA Kit; Glutamate decarboxylase 67 kDa isoform ELISA Kit; Glutamate decarboxylase; brain; 67-KD ELISA Kit; OTTHUMP00000041054 ELISA Kit; OTTHUMP00000041055 ELISA Kit; OTTHUMP00000228572 ELISA Kit; SCP ELISA Kit
缩写：GAD1
Uniprot No.：Q99259
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human GAD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 90-98 | |||||
| 1:2 | Average % | 86 | ||||
| Range % | 82-93 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 90-100 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 87-96 | |||||
回收率：
| The recovery of human GAD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 85-95 | ||||
| EDTA plasma (n=4) | 99 | 95-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.5122.4582.4852.357101.6781.7881.7331.60551.1051.1901.1481.0202.50.6850.7100.6980.5701.250.4740.4650.4700.3420.6250.3400.3210.3310.2030.3120.2400.2210.2310.10300.1250.1310.128 | ||||||
货期：3-5 working days
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