首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠血管内皮细胞生长因子D(VEGF-D)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的Vegfd含量。Vegfd（血管内皮生长因子 D）是一种重要的血管生成调节因子。其背景在于参与多种生理与病理过程，如血管和淋巴管生成。研究机制上，它可与血管内皮生长因子受体结合，激活下游信号通路，调控细胞增殖、迁移等，在肿瘤转移等疾病中作用受关注。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于实验动物模型（如肿瘤、缺血性损伤等）中VEGF - D的动态监测，可兼容多种生物样本类型，为研究组织微环境变化、药物干预效果或基因表达调控提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Vegfd ELISA kit; FigfVascular endothelial growth factor D ELISA kit; VEGF-D ELISA kit; c-Fos-induced growth factor ELISA kit; FIGF ELISA kit
缩写：FIGF
Uniprot No.：O35251
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat VEGF-D in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 87 | |||||
| Range % | 83-92 | ||||||
| 1:2 | Average % | 101 | |||||
| Range % | 97-105 | ||||||
| 1:4 | Average % | 91 | |||||
| Range % | 85-97 | ||||||
| 1:8 | Average % | 97 | |||||
| Range % | 91-103 | ||||||
回收率：
| The recovery of rat VEGF-D spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 91 | 86-95 | |||||
| EDTA plasma (n=4) | 98 | 94-102 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected201.9321.8371.8851.738101.4561.5661.5111.36451.0881.0421.0650.9182.50.7130.7070.7100.5631.250.4920.4810.4870.3400.6250.3580.3420.3500.2030.3120.2720.2780.2750.12800.1480.1460.147 | |||||||
货期：3-5 working days
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