首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠角化细胞生长因子(KGF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的FGF7含量。FGF7即成纤维细胞生长因子 7，属于FGF家族。它在胚胎发育、组织修复等过程发挥重要作用，可促进上皮细胞的增殖、迁移等。其研究机制主要围绕与受体结合激活下游信号通路，在肿瘤、皮肤疾病等领域是潜在治疗靶点。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于皮肤损伤修复机制、上皮细胞再生调控、药物干预效果评估等基础研究领域，为探索KGF在组织稳态维持及疾病模型中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Fgf7 ELISA Kit; Fgf-7 ELISA Kit; KgfFibroblast growth factor 7 ELISA Kit; FGF-7 ELISA Kit; Heparin-binding growth factor 7 ELISA Kit; HBGF-7 ELISA Kit; Keratinocyte growth factor ELISA Kit
缩写：FGF7
Uniprot No.：Q02195
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat KGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 81-95 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 89-101 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 87-96 | |||||
| 1:8 | Average % | 86 | ||||
| Range % | 80-93 | |||||
回收率：
| The recovery of rat KGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 100 | 94-105 | ||||
| EDTA plasma (n=4) | 95 | 89-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.7302.7752.7532.62110002.2262.4032.3152.1835001.5001.6101.5551.4232500.8370.8900.8640.7321250.4280.4320.4300.29862.50.2210.2350.2280.09631.20.1670.1580.1630.03100.1300.1330.132 | ||||||
货期：3-5 working days
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