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Rat Factor-related Apoptosis ligand,FASL ELISA Kit

  • 中文名: 大鼠凋亡相关因子配体(FASL) ELISA Kit
货号: IPD-E07325r
Price: ¥2500
数量:
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产品详情

  • 产品描述:大鼠凋亡相关因子配体(FASL)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的FASLG含量。FASLG即Fas配体,是TNF超家族成员。在细胞凋亡调控等方面有重要作用。研究机制上,FASLG可与Fas受体结合,激活凋亡信号通路,诱导靶细胞凋亡,在免疫调节、肿瘤免疫逃逸等研究领域受关注。试剂盒检测范围为31.25 pg/mL-2000 pg/mL,适用于科研领域对细胞凋亡机制、免疫相关疾病模型或药物干预下 FASL 表达水平变化的研究,例如探索肿瘤微环境、自身免疫性疾病或神经退行性病变中的凋亡调控网络,为相关基础研究提供可靠的数据支持本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。

  • 别名:Faslg; Apt1Lg1; Cd95l; Fasl; Tnfsf6; Tumor necrosis factor ligand superfamily member 6; CD95 ligand; CD95-L; Fas antigen ligand; Fas ligand; FasL; CD antigen CD178

  • 缩写:FASLG

  • Uniprot No.:P36940

  • 种属:Rattus norvegicus (Rat)

  • 样本类型:serum, plasma, tissue homogenates

  • 检测范围:31.25 pg/mL-2000 pg/mL

  • 灵敏度:7.8 pg/mL

  • 反应时间:1-5h

  • 样本体积:50-100ul

  • 检测波长:450 nm

  • 研究领域:Cell Biology

  • 测定原理:quantitative

  • 测定方法:Sandwich

  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%


    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%


    Three samples of known concentration were tested in twenty assays to assess.








  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of rat FASL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    SampleSerum(n=4)
    1:1Average %89
    Range %85-93
    1:2Average %88
    Range %82-94
    1:4Average %101
    Range %95-104
    1:8Average %92
    Range %89-96
  • 回收率:

    The recovery of rat FASL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5)9389-97
    EDTA plasma (n=4)8781-92














  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected20002.2122.4032.3082.16610001.6471.5541.6011.4595001.0050.9880.9970.8552500.5920.5870.5900.4481250.4130.4020.4080.26662.50.2720.2640.2680.12631.250.1880.1920.1900.04800.1430.1410.142






  • 货期:3-5 working days

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