首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠肾上腺素(EPI)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的EPI含量。大鼠肾上腺素（EPI）是肾上腺髓质分泌的儿茶酚胺激素，参与调节心血管、代谢和呼吸系统功能。其作用机制涉及激活α和β肾上腺素能受体，调节心率、血压、血糖和脂肪分解等生理过程。试剂盒检测范围为1 pg/mL-200 pg/mL，该产品可满足神经生物学、药理学及内分泌学等领域的基础研究需求，例如探索应激状态下激素释放机制、心血管疾病模型中交感神经活性评估，或代谢综合征相关动物实验的EPI动态监测。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：EPI
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：1 pg/mL-200 pg/mL
灵敏度：0.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat EPI in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 98 | ||||
| Range % | 90-103 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 85-96 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 89-99 | |||||
| 1:8 | Average % | 85 | ||||
| Range % | 80-90 | |||||
回收率：
| The recovery of rat EPI spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 91 | 85-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 2000.2030.2020.203 500.3780.3510.365 160.5810.5370.559 40.9451.0120.979 11.4161.4931.455 02.2412.3672.304 | ||||||
货期：3-5 working days
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