| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | SCAR3; WAVE3; Brush-1 |
| WB Predicted band size | 55 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human WASF3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于WASF3抗体的3篇参考文献及其摘要概括：
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1. **文献名称**: *WASF3 regulates mitochondrial dynamics and metabolic reprogramming in breast cancer*
**作者**: Sossey-Alaoui K, et al.
**摘要**: 该研究利用WASF3特异性抗体，发现WASF3通过调控线粒体分裂蛋白Drp1的活性，促进乳腺癌细胞的代谢重编程(增强糖酵解)，从而驱动肿瘤侵袭和转移。
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2. **文献名称**: *WAVE3 facilitates EMT and metastasis through Akt/GSK-3β/Snail signaling in hepatocellular carcinoma*
**作者**: Teng Y, et al.
**摘要**: 研究使用WASF3(WAVE3)抗体进行免疫组化和Western blot分析，证明WASF3通过激活Akt/GSK-3β/Snail通路诱导上皮-间质转化(EMT)，促进肝癌细胞转移。
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3. **文献名称**: *Phosphorylation of WASF3 promotes its nuclear translocation and oncogenic function in prostate cancer*
**作者**: Sossey-Alaoui K, et al.
**摘要**: 该文献通过磷酸化特异性抗体及总WASF3抗体的联合应用，揭示WASF3在酪氨酸残基磷酸化后易位至细胞核，调控前列腺癌基因表达并促进去势抵抗性进展。
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**备注**：上述文献均通过WASF3抗体进行蛋白表达定位、功能机制或翻译后修饰研究，涵盖乳腺癌、肝癌、前列腺癌等模型。如需具体抗体品牌或实验细节，需进一步查阅方法学部分。
The WASF3 antibody is a crucial tool for studying the WASF3 protein (also known as WAVE3), a member of the Wiskott-Aldrich syndrome protein family (WASF) involved in actin cytoskeleton reorganization. WASF3 regulates cell motility and invasion by activating the Arp2/3 complex, which drives branched actin network formation. Overexpression of WASF3 is linked to cancer metastasis, particularly in breast, prostate, and colorectal cancers, making it a focus in oncology research. The antibody enables detection and quantification of WASF3 expression in experimental models, aiding investigations into its role in epithelial-mesenchymal transition (EMT), tumor progression, and signaling pathways like Rac1 and PI3K/AKT.
WASF3 antibodies are widely used in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to assess protein localization and expression levels in tissues or cell lines. Recent studies highlight its potential as a biomarker for aggressive cancers and a therapeutic target. However, cross-reactivity with other WASF family members (e.g., WASF1/WAVE1) requires validation via knockout controls or epitope-specific antibodies. Research using WASF3 antibodies has advanced understanding of metastatic mechanisms and may inform strategies to inhibit cancer cell dissemination. Commercial availability of monoclonal and polyclonal variants ensures broad applicability, though optimization for specific experimental conditions remains essential.
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