Recombinant E. coli pykF protein

中文名： 丙酮酸激酶I(pykF)重组蛋白
别名： pykF;Pyruvate kinase I

货号: PA1000-2598

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>95%SDS-PAGE.
种属	Escherichia coli
靶点	pykF
Uniprot No	P0AD61
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	1-470aa
氨基酸序列	MGSSHHHHHH SSGLVPRGSH MGSHMKKTKI VCTIGPKTES EEMLAKMLDA GMNVMRLNFS HGDYAEHGQR IQNLRNVMSK TGKTAAILLD TKGPEIRTMK LEGGNDVSLK AGQTFTFTTD KSVIGNSEMV AVTYEGFTTD LSVGNTVLVD DGLIGMEVTA IEGNKVICKV LNNGDLGENK GVNLPGVSIA LPALAEKDKQ DLIFGCEQGV DFVAASFIRK RSDVIEIREH LKAHGGENIH IISKIENQEG LNNFDEILEA SDGIMVARGD LGVEIPVEEV IFAQKMMIEK CIRARKVVIT ATQMLDSMIK NPRPTRAEAG DVANAILDGT DAVMLSGESA KGKYPLEAVS IMATICERTD RVMNSRLEFN NDNRKLRITE AVCRGAVETA EKLDAPLIVV ATQGGKSARA VRKYFPDATI LALTTNEKTA HQLVLSKGVV PQLVKEITST DDFYRLGKEL ALQSGLAHKG DVVVMVSGAL VPSGTTNTAS VHVL
预测分子量	53 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于pykF重组蛋白的3篇示例参考文献(注：文献为模拟示例，实际需通过学术数据库验证)：

1. **文献名称**：Cloning and expression of pykF gene in Escherichia coli for enhanced pyruvate production

**作者**：Smith, J.R. et al.

**摘要**：研究报道了通过克隆大肠杆菌pykF基因并构建重组质粒，成功在工程菌中过表达丙酮酸激酶I(PykF)，显著提高了菌株的丙酮酸代谢效率。

2. **文献名称**：Structural analysis of PykF recombinant protein and its allosteric regulation

**作者**：Chen, L. & Tanaka, M.

**摘要**：通过X射线晶体学解析了重组表达的PykF蛋白三维结构，揭示了其受FBP(果糖-1.6-二磷酸)变构激活的分子机制。

3. **文献名称**：Application of PykF-deficient strains in lactic acid fermentation

**作者**：Wang, Y. et al.

**摘要**：构建了pykF基因敲除的大肠杆菌突变株，结合重组表达优化后的PykF变体，实现了乳酸合成途径的代谢流定向调控。

建议通过PubMed、Web of Science或Google Scholar搜索关键词 "pykF recombinant protein" 或 "pykF overexpression" 获取真实文献。

背景信息

**Background of PykF Recombinant Protein**

PykF, encoded by the *pykF* gene in *Escherichia coli*, is one of two isoforms of pyruvate kinase (the other being PykA) that catalyzes the final step in glycolysis, converting phosphoenolpyruvate (PEP) and ADP to pyruvate and ATP. This reaction is critical for energy production and carbon flux regulation, linking glycolysis with downstream metabolic pathways like the tricarboxylic acid (TCA) cycle or fermentation. PykF activity is tightly regulated by cellular energy status and carbon source availability, often modulated by allosteric effectors such as fructose-1.6-bisphosphate (FBP), which activates the enzyme, or ATP, which inhibits it.

Recombinant PykF protein is produced via genetic engineering, typically by cloning the *pykF* gene into expression vectors (e.g., plasmid systems) and overexpressing it in bacterial hosts like *E. coli*. Purification techniques (e.g., affinity chromatography) yield high-purity PykF for research or industrial applications. Its recombinant form is widely used to study glycolytic regulation, enzyme kinetics, and metabolic adaptations in microbes. Additionally, PykF serves as a model enzyme for structural biology (e.g., crystallography) to elucidate mechanisms of allosteric regulation and substrate binding.

In biotechnology, PykF has applications in metabolic engineering, where modulating its activity can optimize carbon flow toward desired products, such as biofuels or biochemicals. Its thermostable or engineered variants are also explored for industrial biocatalysis. Research on PykF recombination contributes to understanding microbial physiology, energy metabolism, and the development of synthetic biology tools. Overall, PykF recombinant protein remains a vital resource in both basic science and applied biotechnological innovations.