首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠脱氢表雄酮硫酸酯(DHEA-S)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的DHEA-S含量。DHEA-S即硫酸脱氢表雄酮，是肾上腺皮质分泌的一种激素前体。它在人体生理活动中起重要作用，如影响代谢、免疫等。研究机制上，其可转化为雄激素和雌激素，参与调节多种生理功能，对它的研究有助于深入了解人体内分泌及相关疾病机制。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，本产品适用于动物模型中的激素水平监测、代谢性疾病机制研究、应激生理学分析等科研场景，例如通过检测大鼠血清/血浆DHEA - S浓度，探究肾上腺皮质功能状态或激素相关疾病的分子调控网络，为内分泌领域的机制研究提供高效工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：DHEA-S
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat DHEA-S in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 87 | ||||
| Range % | 81-92 | |||||
| 1:200 | Average % | 96 | ||||
| Range % | 90-100 | |||||
| 1:400 | Average % | 95 | ||||
| Range % | 91-104 | |||||
| 1:800 | Average % | 92 | ||||
| Range % | 84-98 | |||||
回收率：
| The recovery of rat DHEA-S spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 85-97 | ||||
| EDTA plasma (n=4) | 97 | 91-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.1032.2042.1542.01210001.9141.9211.9181.7765001.3771.4051.3911.2492500.7530.7620.7580.6161250.4340.4630.4490.30762.50.2910.3010.2960.15431.250.2170.2340.2260.08400.1410.1430.142 | ||||||
货期：3-5 working days
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