首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠脱氢表雄酮(DHEA)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的DHEA含量。DHEA即脱氢表雄酮，是人体内重要的激素前体。它由肾上腺皮质、性腺及大脑分泌，参与体内多种生理过程。研究机制上，其可调节神经、免疫等系统功能，还与代谢、衰老等密切相关，目前在代谢性疾病、抗衰老等领域有广泛研究。试剂盒检测范围为0.05 ng/mL-10 ng/mL，该产品适用于动物模型中的内分泌研究，如代谢综合征、多囊卵巢综合征的机制探索，或用于评估药物干预对肾上腺功能的影响。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：DHEA
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：0.05 ng/mL-10 ng/mL
灵敏度：0.025 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat DHEA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 102 | ||||
| Range % | 98-107 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 88-96 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 85-93 | |||||
| 1:8 | Average % | 100 | ||||
| Range % | 97-104 | |||||
回收率：
| The recovery of rat DHEA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 85-92 | ||||
| EDTA plasma (n=4) | 94 | 88-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average100.1620.1580.1602.50.3070.2980.3030.80.4870.5040.4960.20.8010.8100.8060.051.2241.3051.26502.0001.8991.950 | ||||||
货期：3-5 working days
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