纯度 | >95%SDS-PAGE. |
种属 | Human |
靶点 | PSMG4 |
Uniprot No | Q5JS54 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 1-123aa |
氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMEGLVVAAGGDVSLHNFSARLWEQLVHFHV MRLTDSLFLWVGATPHLRNLAVAMCSRYDSIPVSTSLLGDTSDTTSTGLA QRLARKTNKQVFVSYNLQNTDSNFALLVENRIKEEMEAFPEKF |
预测分子量 | 16 kDa |
蛋白标签 | His tag N-Terminus |
缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是假设性参考文献格式示例(PSMG4相关研究较少,以下内容为模拟,请根据实际文献调整):
1. **标题**:The role of PSMG4 as a proteasome assembly chaperone in mammalian cells
**作者**:Smith J, et al.
**摘要**:本研究揭示了PSMG4作为蛋白酶体组装伴侣蛋白的功能,通过重组表达技术分析了其与蛋白酶体亚基的相互作用机制,证实了PSMG4在维持蛋白酶体活性中的关键作用。
2. **标题**:Crystal structure of recombinant human PSMG4 and its implications for chaperone activity
**作者**:Chen L, et al.
**摘要**:报道了人源PSMG4重组蛋白的晶体结构,通过体外实验阐明了其通过特定结构域介导蛋白酶体亚基组装的分子机制。
3. **标题**:PSMG4 knockout cell lines generated via CRISPR exhibit impaired proteasome biogenesis
**作者**:Wang Y, et al.
**摘要**:利用重组PSMG4蛋白回补实验,验证了PSMG4缺失导致细胞蛋白酶体组装缺陷的表型,证明其作为分子伴侣的必需性。
4. **标题**:High-yield expression and purification of recombinant PSMG4 in E. coli for functional studies
**作者**:Kim T, et al.
**摘要**:开发了PSMG4重组蛋白在大肠杆菌中的高效可溶性表达及纯化方案,为后续生化研究提供了材料基础。
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**注意**:以上内容为模拟生成,实际文献需通过PubMed/Google Scholar等平台检索关键词(如"PSMG4"、"Proteasome Assembly Chaperone 4"或"PAC4")。若需真实文献,建议补充具体研究背景(如疾病关联或分子机制方向)。
**Background of PSMG4 Recombinant Protein**
PSMG4 (Proteasome Assembly Chaperone 4), also known as PAC4. is a critical molecular chaperone involved in the assembly of the 20S proteasome, a multicatalytic protease complex responsible for degrading ubiquitinated proteins in eukaryotic cells. The proteasome plays a vital role in maintaining cellular homeostasis by regulating protein turnover, which impacts processes such as cell cycle progression, stress response, and immune regulation. PSMG4 specifically facilitates the correct assembly of α- and β-subunits into the proteasome's core structure, ensuring its functional integrity.
Recombinant PSMG4 is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to produce purified, biologically active protein for research. Its recombinant form retains the ability to interact with proteasome subunits, making it a valuable tool for studying proteasome biogenesis, dynamics, and related pathologies. Dysregulation of proteasome activity is linked to neurodegenerative diseases, cancer, and autoimmune disorders, underscoring PSMG4's relevance in therapeutic exploration.
Structurally, PSMG4 contains conserved domains that mediate binding to proteasome precursors. Studies utilizing recombinant PSMG4 have elucidated its role in preventing premature aggregation of subunits and promoting stepwise assembly. Additionally, it serves as a reagent in high-throughput screens for modulators of proteasome activity, aiding drug development.
The production of recombinant PSMG4 often includes affinity tags (e.g., His-tag) for purification, ensuring high yield and purity. Its application spans *in vitro* reconstitution assays, structural studies (e.g., cryo-EM), and investigations into diseases associated with proteasomal dysfunction. Overall, PSMG4 recombinant protein is pivotal for advancing mechanistic and translational research in proteostasis and related biomedical fields.
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