首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述:大鼠生长调节致癌基因α/黑素瘤生长刺激因子(GROα/CXCL1/MGSA)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的CXCL1含量。CXCL1 是重要的趋化因子。其背景上,在炎症反应等生理病理过程中发挥作用。研究机制方面,它能通过与相应受体结合,招募免疫细胞到特定组织部位,在肿瘤发生发展、血管生成等过程中也有参与,相关研究有助于疾病治疗探索。试剂盒检测范围为125 pg/mL-8000 pg/mL,适用于科研领域对生长调节致癌基因α(GROα/CXCL1/MGSA)的精准分析,为炎症性疾病机制研究、肿瘤微环境分析或药物干预效果评估提供可靠工具,适用于实验性炎症模型(如急性肺损伤、关节炎)、肿瘤生长研究或免疫调控相关的大鼠体内实验数据获取,帮助研究者深入探索GROα在病理生理过程中的动态变化及其调控网络本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:Cxcl1 ELISA Kit; Cinc1 ELISA Kit; Gro ELISA Kit; Scyb1Growth-regulated alpha protein ELISA Kit; C-X-C motif chemokine 1 ELISA Kit; Cytokine-induced neutrophil chemoattractant 1 ELISA Kit; CINC-1 ELISA Kit; Platelet-derived growth factor-inducible protein KC ELISA Kit
缩写:CXCL1
Uniprot No.:P14095
种属:Rattus norvegicus (Rat)
样本类型:serum, plasma, tissue homogenates
检测范围:125 pg/mL-8000 pg/mL
灵敏度:31.25 pg/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Immunology
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
Inter-assay Precision (Precision between assays): CV%<10% | |||||||
Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of rat GROα /MGSA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
Sample | Serum(n=4) | ||||||
1:1 | Average % | 91 | |||||
Range % | 86-95 | ||||||
1:2 | Average % | 106 | |||||
Range % | 101-112 | ||||||
1:4 | Average % | 93 | |||||
Range % | 87-96 | ||||||
1:8 | Average % | 93 | |||||
Range % | 86-98 |
回收率:
The recovery of rat GROα /MGSA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
Sample Type | Average % Recovery | Range | |||||
Serum (n=5) | 94 | 89-98 | |||||
EDTA plasma (n=4) | 99 | 96-104 | |||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
pg/mlOD1OD2AverageCorrected80002.4542.5892.5222.39640002.0122.1802.0961.97020001.4451.4961.4711.34510000.7840.8090.7970.6715000.4330.4410.4370.3112500.2360.2490.2430.1171250.1730.1790.1760.05000.1250.1270.126 |
货期:3-5 working days
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