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产品描述：人细胞毒性T淋巴细胞相关抗原4(CTLA-4/CD152)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CTLA4含量。CTLA4（细胞毒性T淋巴细胞相关抗原4）是一种免疫检查点蛋白，主要表达在T细胞表面，通过与B7分子结合传递抑制信号，抑制T细胞活化。CTLA4的研究机制主要聚焦于其如何通过抑制T细胞活性来调节免疫反应，以及如何通过抗体药物阻断其功能以增强抗肿瘤免疫。试剂盒检测范围为125 pg/mL-8000 pg/mL，适用于基础科研中探索CTLA - 4在肿瘤微环境、免疫细胞功能调控中的作用，或用于评估免疫调节药物、抗体药物对CTLA - 4表达水平的影响研究。为研究CTLA - 4在不同生理/病理状态下的表达差异提供灵活选择，特别适用于肿瘤免疫治疗机制、免疫相关性疾病模型构建等研究场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CTLA4; CD152; Cytotoxic T-lymphocyte protein 4; Cytotoxic T-lymphocyte-associated antigen 4; CTLA-4; CD antigen CD152
缩写：CTLA4
Uniprot No.：P16410
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：125 pg/mL-8000 pg/mL
灵敏度：31.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CTLA-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 99 | ||||
| Range % | 96-102 | |||||
| 1:2 | Average % | 87 | ||||
| Range % | 84-93 | |||||
| 1:4 | Average % | 111 | ||||
| Range % | 107-115 | |||||
| 1:8 | Average % | 104 | ||||
| Range % | 100-108 | |||||
回收率：
| The recovery of human CTLA-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 85 | 81-89 | ||||
| EDTA plasma (n=4) | 92 | 87-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 80002.3032.3982.3512.249 40001.4201.4681.4441.342 20000.7450.7040.7250.623 10000.4230.4090.4160.314 5000.2850.2980.2920.190 2500.2220.2320.2270.125 1250.1610.1680.1650.063 00.1030.1010.102 | ||||||
货期：3-5 working days
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