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产品描述：人胶原蛋白三螺旋重复包含蛋白1(CTHRC1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CTHRC1含量。CTHRC1 是一种与多种生理病理过程相关的靶点。研究表明它在细胞增殖、迁移和血管生成等方面发挥作用。其过度表达与肿瘤的发生、发展及转移密切相关，调控 CTHRC1 活性或表达水平，有望为肿瘤等疾病治疗提供新策略。试剂盒检测范围为6.25 ng/mL-400 ng/mL，可用于探索CTHRC1在疾病模型（如肝纤维化、肿瘤转移）中的功能机制，或评估药物干预、基因编辑后该蛋白的动态变化；为体外研究CTHRC1的生物学作用及其分子调控网络提供精准的检测方案本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Collagen triple helix repeat containing 1 ELISA Kit; Collagen triple helix repeat containing protein 1 ELISA Kit; Collagen triple helix repeat-containing protein 1 ELISA Kit; CTHR1_HUMAN ELISA Kit; Cthrc1 ELISA Kit; NMTC1 protein ELISA Kit; Protein NMTC1 ELISA Kit
缩写：CTHRC1
Uniprot No.：Q96CG8
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：6.25 ng/mL-400 ng/mL
灵敏度：1.56 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CTHRC1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 80-89 | |||||
| 1:2 | Average % | 91 | ||||
| Range % | 88-96 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 87-96 | |||||
回收率：
| The recovery of human CTHRC1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 99 | 92-104 | ||||
| EDTA plasma (n=4) | 92 | 89-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected4002.4452.3722.4092.2172001.8721.9501.9111.7191001.4011.4181.4101.218500.8920.8630.8780.686250.5850.5440.5650.37312.50.4030.3890.3960.2046.250.2920.2790.2860.09400.1940.1900.192 | ||||||
货期：3-5 working days
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