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产品描述：人结缔组织生长因子(CTGF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CTGF含量。CTGF（结缔组织生长因子）是一种多功能的细胞因子，主要在组织修复和纤维化过程中发挥作用。它作为TGF-β的下游效应分子，能促进细胞增殖、胶原合成和ECM沉积，在多种纤维化疾病如肠纤维化、肝纤维化中起关键作用。研究CTGF的信号通路和调控机制，有助于开发针对纤维化疾病的新治疗方法。试剂盒检测范围为3.75 pg/mL-120 pg/mL，科研人员可通过探究纤维化疾病（如肝纤维化、肺纤维化）、心血管重构、糖尿病肾病等模型的分子机制，或用于评估药物干预对CTGF信号通路的影响。适用于体外实验中对CTGF表达水平的动态监测。为CTGF相关基础研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CCN 2 ELISA Kit; CCN family member 2 ELISA Kit; CCN2 ELISA Kit; Connective tissue growth factor ELISA Kit; Ctgf ELISA Kit; CTGF_HUMAN ELISA Kit; Hcs 24 ELISA Kit; Hcs24 ELISA Kit; Hypertrophic chondrocyte specific protein 24 ELISA Kit; Hypertrophic chondrocyte-specific gene product 24 ELISA Kit; Hypertrophic chondrocyte-specific protein 24 ELISA Kit; IBP-8 ELISA Kit; IGF-binding protein 8 ELISA Kit; IGFBP-8 ELISA Kit; IGFBP8 ELISA Kit; Insulin like Growth Factor Binding Protein 8 ELISA Kit; Insulin-like growth factor-binding protein 8 ELISA Kit; MGC102839 ELISA Kit; NOV 2 ELISA Kit; NOV2 ELISA Kit
缩写：CTGF
Uniprot No.：P29279
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：3.75 pg/mL-120 pg/mL
灵敏度：1.875 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CTGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 92-103 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 86-96 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 84-96 | |||||
| 1:8 | Average % | 86 | ||||
| Range % | 80-90 | |||||
回收率：
| The recovery of human CTGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 88 | 84-90 | ||||
| EDTA plasma (n=4) | 92 | 88-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 1201.8511.9541.9031.859 601.0781.0871.0831.039 26.250.5130.5020.5080.464 11.250.2550.2680.2620.218 3.750.1190.1160.1180.074 00.0430.0440.044 | ||||||
货期：3-5 working days
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