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产品描述：人心肌营养素1(CT-1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的CTF1含量。试剂盒检测范围为7.8 pg/mL-500 pg/mL，适用于心血管疾病基础研究，如心肌损伤模型、药物干预实验中的CT-1分泌监测，亦可分析临床前动物实验或体外细胞模型中CT-1在组织修复、细胞增殖调控等过程的生物学功能。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Cardiophin 1 ELISA Kit; Cardiophin1 ELISA Kit; Cardiotrophin I ELISA Kit; Cardiotrophin-1 ELISA Kit; Cardiotrophin1 ELISA Kit; CardiotrophinI ELISA Kit; CT 1 ELISA Kit; CT-1 ELISA Kit; CT1 ELISA Kit; CTF 1 ELISA Kit; Ctf1 ELISA Kit; CTF1_HUMAN ELISA Kit
缩写：CTF1
Uniprot No.：Q16619
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：7.8 pg/mL-500 pg/mL
灵敏度：1.95 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CT-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 96 | |||||
| Range % | 87-105 | ||||||
| 1:2 | Average % | 95 | |||||
| Range % | 90-102 | ||||||
| 1:4 | Average % | 89 | |||||
| Range % | 83-97 | ||||||
| 1:8 | Average % | 95 | |||||
| Range % | 85-102 | ||||||
回收率：
| The recovery of human CT-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 97 | 92-99 | |||||
| EDTA plasma (n=4) | 95 | 87-102 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected5002.1892.1722.1812.0842501.6171.6391.6281.5311251.0521.0341.0430.94662.50.5680.5920.5800.48331.250.3070.3220.3150.21815.630.1870.1900.1890.0927.80.1360.1330.1350.03800.0950.0990.097 | |||||||
货期：3-5 working days
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