首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒

Human Macrophage Colony-Stimulating Factor,M-CSF ELISA kit

  • 中文名： 人巨噬细胞集落刺激因子(M-CSF)ELISA Kit
货号: IPD-E04658h
Price： ￥2500
数量：
大包装询价

产品详情

  • 产品描述：
    人巨噬细胞集落刺激因子(M-CSF)酶联免疫试剂盒，为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CSF1含量。CSF1，即巨噬细胞集落刺激因子，是一种分泌型细胞因子，主要诱导造血干细胞分化为巨噬细胞。其研究机制涉及CSF1与CSF1R的相互作用，调节巨噬细胞的功能和存活，与肿瘤、炎症等多种疾病的发生发展密切相关。试剂盒检测范围为0.156 ng/ml- 10 ng/ml，灵敏度为0.039 ng/ml。支持科研人员高效开展M-CSF相关的基础研究，例如探索炎症性疾病中巨噬细胞活化机制、评估肿瘤模型内免疫微环境变化，或分析药物干预对M-CSF表达的影响等实验需求本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Colony stimulating factor 1 (macrophage) ELISA Kit; Colony stimulating factor 1 ELISA Kit; Colony stimulating factor macrophage specific ELISA Kit; CSF 1 ELISA Kit; CSF-1 ELISA Kit; CSF1 ELISA Kit; CSF1_HUMAN ELISA Kit; Csfm ELISA Kit; Lanimostim ELISA Kit; M CSF ELISA Kit; M-CSF ELISA Kit; Macrophage Colony Stimulating Factor 1 ELISA Kit; Macrophage colony stimulating factor ELISA Kit; MCSF ELISA Kit; MGC31930 ELISA Kit; Processed macrophage colony-stimulating factor 1 ELISA Kit
  • 缩写：
    CSF1
  • Uniprot No.：
    P09603
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.156 ng/ml - 10 ng/ml
  • 灵敏度：
    0.039 ng/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human M-CSF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    84


    Range %

    80-89


    1:2

    Average %

    89


    Range %

    84-95


    1:4

    Average %

    98


    Range %

    94-101


    1:8

    Average %

    92


    Range %

    87-96


  • 回收率：

    The recovery of human M-CSF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    98

    90-105


    EDTA plasma (n=4)

    92

    87-97


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    10

    2.793

    2.672

    2.733

    2.584


    5

    2.341

    2.306

    2.324

    2.175


    2.5

    1.827

    1.724

    1.776

    1.627


    1.25

    1.269

    1.329

    1.299

    1.150


    0.625

    0.914

    0.922

    0.918

    0.769


    0.312

    0.594

    0.630

    0.612

    0.463


    0.156

    0.309

    0.289

    0.299

    0.150


    0

    0.152

    0.145

    0.149




  • 货期：
    3-5 working days

客户数据及评论

折叠内容

大包装询价

×