首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人α晶状体蛋白B链(CRYAB)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的CRYAB含量。CRYAB即αB-晶状体蛋白，是小热休克蛋白家族成员，广泛表达于多种组织。它具有分子伴侣功能，能保护细胞抵抗应激损伤。研究机制方面，其参与细胞的凋亡调控、抗氧化应激等过程，异常表达与多种疾病如神经系统疾病、心肌病等相关。试剂盒检测范围为12.5 pg/mL-800 pg/mL，适用于探索CRYAB在疾病模型中的表达调控机制、评估其在特定病理条件下的生物标志物价值，或用于分子伴侣功能相关的基础研究。操作流程经过优化，支持多样本类型同步检测，为研究晶状体蛋白相关细胞应激反应、退行性疾病分子机制及药物干预效果提供可靠工具，适用于生命科学、基础医学等领域的体外实验研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：AACRYA ELISA Kit; Alpha B crystallin ELISA Kit; Alpha crystallin B chain ELISA Kit; Alpha(B)-crystallin ELISA Kit; Alpha-crystallin B chain ELISA Kit; CRYA2 ELISA Kit; Cryab ELISA Kit; CRYAB_HUMAN ELISA Kit; Crystallin alpha B ELISA Kit; Crystallin alpha polypeptide 2 ELISA Kit; CTPP2 ELISA Kit; Heat shock 20 kD like protein ELISA Kit; Heat shock protein beta 5 ELISA Kit; Heat shock protein beta-5 ELISA Kit; HspB5 ELISA Kit; Renal carcinoma antigen NY REN 27 ELISA Kit; Renal carcinoma antigen NY-REN-27 ELISA Kit; Rosenthal fiber component ELISA Kit
缩写：CRYAB
Uniprot No.：P02511
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：12.5 pg/mL-800 pg/mL
灵敏度：3.12 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CRYAB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 85-86 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 92-101 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 84-93 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 90-99 | |||||
回收率：
| The recovery of human CRYAB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-101 | ||||
| EDTA plasma (n=4) | 84 | 80-88 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 8002.4562.3272.3922.299 4001.6891.6211.6551.562 2001.0521.0131.0330.940 1000.4970.5070.5020.409 500.3040.3290.3170.224 250.1810.1970.1890.096 12.50.1490.1400.1450.052 00.0950.0910.093 | ||||||
货期：3-5 working days
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