首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人促肾上皮质激素释放激素(CRH)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CRH含量。CRH即促肾上腺皮质激素释放激素，是应激反应重要调节因子。其在调节神经内分泌、免疫等系统中起关键作用。研究机制上，它主要通过与受体结合，激活下游信号通路，影响神经递质释放、内分泌激素合成与分泌等，广泛参与应激相关疾病病理过程。试剂盒检测范围为1 ng/mL-40 ng/mL，适用于科研领域中对CRH表达水平的精准定量，例如研究应激相关疾病模型中的激素分泌动态、神经内分泌调控机制、组织局部CRH分布特征等。为探索CRH在生理病理过程中的作用提供了可靠工具，适用于神经科学、内分泌学及基础医学研究中的体外实验需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CRHCorticoliberin ELISA Kit; Corticotropin-releasing factor ELISA Kit; CRF ELISA Kit; Corticotropin-releasing hormone ELISA Kit
缩写：CRH
Uniprot No.：P06850
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：1 ng/mL-40 ng/mL
灵敏度：1 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CRH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 98 | ||||
| Range % | 94-103 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 90-100 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 88-98 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 90-101 | |||||
回收率：
| The recovery of human CRH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 99 | 95-102 | ||||
| EDTA plasma (n=4) | 96 | 91-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 01.9111.8381.875 11.3081.2971.303 31.0251.0051.015 9.20.7510.7360.744 200.5700.5430.557 400.3510.3420.347 | ||||||
货期：3-5 working days
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