首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人cAMP反应元件结合蛋白(CREB)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CREB1含量。CREB1是细胞内重要的转录调节因子，与多种生理和病理过程相关。在神经可塑性、细胞存活及代谢调控等方面发挥关键作用。研究机制主要聚焦于其磷酸化激活途径，及其如何与特定DNA序列结合调控基因表达。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于探究CREB在神经退行性疾病、代谢综合征等病理模型中的表达变化，也可用于药物干预或基因编辑实验中的靶点效应评估。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Active transcription factor CREB ELISA Kit; cAMP response element binding protein 1 ELISA Kit; cAMP response element binding protein ELISA Kit; cAMP responsive element binding protein 1 ELISA Kit; cAMP-responsive element-binding protein 1 ELISA Kit; CREB ELISA Kit; CREB-1 ELISA Kit; CREB1 ELISA Kit; CREB1_HUMAN ELISA Kit; Cyclic AMP-responsive element-binding protein 1 ELISA Kit; MGC9284 ELISA Kit; OTTHUMP00000163864 ELISA Kit; OTTHUMP00000163865 ELISA Kit; OTTHUMP00000206660 ELISA Kit; OTTHUMP00000206662 ELISA Kit; OTTHUMP00000206667 ELISA Kit; Transactivator protein ELISA Kit
缩写：CREB1
Uniprot No.：P16220
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CREB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 92 | ||||
| Range % | 87-96 | |||||
| 1:10 | Average % | 89 | ||||
| Range % | 85-94 | |||||
| 1:20 | Average % | 95 | ||||
| Range % | 89-98 | |||||
| 1:40 | Average % | 102 | ||||
| Range % | 98-105 | |||||
回收率：
| The recovery of human CREB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 93-102 | ||||
| EDTA plasma (n=4) | 86 | 80-92 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.1572.2542.2062.104101.7151.8121.7641.66251.2671.2841.2761.1742.50.8570.8630.8600.7581.250.5230.5320.5280.4260.6250.3430.3510.3470.2450.3120.2060.2130.2100.10800.1030.1010.102 | ||||||
货期：3-5 working days
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