首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人羧基肽酶N催化链(CPN1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CPN1含量。CPN1是一种细胞分裂素/嘌呤核糖核苷核苷酶，参与植物生长促进激素细胞分裂素的代谢。研究发现，CPN1存在于细胞壁空间中，通过活化从根部运输的前体，影响水稻穗形成等生长过程，有望应用于提高作物产量。试剂盒检测范围为31.25 mU/mL-2000 mU/mL，适用于体外实验中探索CPN1在炎症反应调控、血管功能异常或代谢性疾病中的分子机制，也可用于评估不同实验条件下（如药物处理或基因干预）生物样本中CPN1的活性动态变化。该产品为研究CPN1在基础科研中的生物学功能及其作为潜在生物标志物的应用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ACBP ELISA Kit; Anaphylatoxin inactivator ELISA Kit; Arginine carboxypeptidase ELISA Kit; Carboxypeptidase N catalytic chain ELISA Kit; Carboxypeptidase N polypeptide 1 50 kD ELISA Kit; Carboxypeptidase N polypeptide 1 ELISA Kit; Carboxypeptidase N small subunit ELISA Kit; Carboxypeptidase N subunit 1 ELISA Kit; CBPN_HUMAN ELISA Kit; CPN ELISA Kit; CPN1 ELISA Kit; Kininase 1 ELISA Kit; Kininase-1 ELISA Kit; Kininase1 ELISA Kit; Lysine carboxypeptidase ELISA Kit; Plasma carboxypeptidase B ELISA Kit; SCPN ELISA Kit; Serum carboxypeptidase N ELISA Kit
缩写：CPN1
Uniprot No.：P15169
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 mU/mL-2000 mU/mL
灵敏度：7.8 mU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CPN1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 90 | |||||
| Range % | 85-95 | ||||||
| 1:2 | Average % | 105 | |||||
| Range % | 101-109 | ||||||
| 1:4 | Average % | 87 | |||||
| Range % | 84-92 | ||||||
| 1:8 | Average % | 95 | |||||
| Range % | 91-99 | ||||||
回收率：
| The recovery of human CPN1 piked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 98 | 93-103 | |||||
| EDTA plasma (n=4) | 92 | 85-97 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| mU/mlOD1OD2AverageCorrected 20001.9982.1122.0551.916 10001.5471.6751.6111.472 5001.1221.1321.1270.988 2500.6830.6880.6860.547 1250.4360.4540.4450.306 62.50.3210.3250.3230.184 31.250.2320.2410.2370.098 00.1380.1400.139 | |||||||
货期：3-5 working days
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