关键信息
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基因名
BCAT2
- 应用
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别名
BCAM; BCT2; PP18; BCATM; ECA40; Placental protein 18; Branched-chain-amino-acid aminotransferase, mitochondrial
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种属
Human
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表达系统
E. coli
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标签
N-His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O15382
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表达区间
Ala107~Val392
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分子量
33kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
BCAT2 (branched-chain amino acid transaminase 2) is an important enzyme that plays a crucial role in the metabolism of branched-chain amino acids (BCAAs) such as leucine, isoleucine, and valine. These amino acids are essential for numerous physiological functions, including protein synthesis, energy production, and the regulation of metabolic pathways. Research has shown that BCAT2 is predominantly expressed in the brain and skeletal muscle, suggesting its vital role in amino acid catabolism and neurotransmitter synthesis. Alterations in BCAT2 activity have been linked to various metabolic disorders, including obesity, diabetes, and neurological diseases. Given its significance, recombinant protein studies of BCAT2 have gained traction, allowing scientists to elucidate its enzymatic mechanisms, identify potential inhibitors, and explore its role in metabolic regulation. Utilizing recombinant DNA technology, researchers can produce BCAT2 in heterologous systems, facilitating detailed biochemical analyses and structural studies. This research is not only pivotal for understanding the enzyme's function but also for developing therapeutic strategies aimed at modulating BCAA metabolism in various health conditions. As a potential target for drug development, BCAT2 offers promising avenues for intervention in metabolic dysfunctions, making it a critical focus in metabolic and neurological research.
