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产品描述：人Corin酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的CORIN含量。试剂盒检测范围为0.625 ng/mL-40 ng/mL，其稳定的检测性能支持心血管疾病模型研究、药物干预效果评估、组织特异性表达分析等科研场景，尤其适用于探究Corin在心肌肥大、心力衰竭等病理过程中的分子机制，或作为体外细胞实验的蛋白表达监测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：activated protease fragment ELISA Kit; ATC 2 ELISA Kit; ATC2 ELISA Kit; Atrial natriuretic peptide-converting enzyme ELISA Kit; Atrial natriuteric peptide converting enzyme ELISA Kit; Corin ELISA Kit; Corin serine peptidase ELISA Kit; CORIN_HUMAN ELISA Kit; CRN ELISA Kit; Heart specific serine proteinase ELISA Kit; Heart specific serine proteinase ATC2 ELISA Kit; Heart-specific serine proteinase ATC2 ELISA Kit; MGC119742 ELISA Kit; Pro ANP convertase ELISA Kit; Pro ANP converting enzyme ELISA Kit; Pro-ANP-converting enzyme ELISA Kit; TMPRSS 10 ELISA Kit; TMPRSS10 ELISA Kit; Transmembrane protease serine 10 ELISA Kit
缩写：CORIN
Uniprot No.：Q9Y5Q5
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human Corin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 87 | |||||
| Range % | 83-95 | ||||||
| 1:2 | Average % | 98 | |||||
| Range % | 94-102 | ||||||
| 1:4 | Average % | 102 | |||||
| Range % | 98-106 | ||||||
| 1:8 | Average % | 95 | |||||
| Range % | 90-100 | ||||||
回收率：
| The recovery of human Corin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 91 | 85-104 | |||||
| EDTA plasma (n=4) | 104 | 88-108 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected 402.5292.6252.5772.425 202.0072.1212.0641.912 101.2351.2451.2401.088 50.7440.7570.7510.599 2.50.4280.4550.4420.290 1.250.2840.2910.2880.136 0.6250.2330.2450.2390.087 00.1500.1540.152 | |||||||
货期：3-5 working days
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