首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人辅酶Q10 (CoQ10)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CoQ10含量。CoQ10即辅酶Q10，是一种存在于人体细胞内的脂溶性醌类化合物，参与细胞能量生成与抗氧化防御。研究机制方面，它在线粒体呼吸链中传递电子、质子，促进ATP合成，还能清除自由基，保护细胞免受氧化损伤。试剂盒检测范围为3.12 ng/mL-50 ng/mL，支持能量代谢研究、疾病模型机制探索（如心肌缺血、帕金森病等）以及膳食补充剂效果评估等实验需求本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：CoQ10
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 ng/mL-50 ng/mL
灵敏度：1.56 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CoQ10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:20 | Average % | 96 | ||||
| Range % | 91-101 | |||||
| 1:40 | Average % | 99 | ||||
| Range % | 93-104 | |||||
| 1:80 | Average % | 92 | ||||
| Range % | 87-96 | |||||
| 1:160 | Average % | 90 | ||||
| Range % | 85-94 | |||||
回收率：
| The recovery of human CoQ10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 84 | 80-88 | ||||
| EDTA plasma (n=4) | 93 | 88-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 500.1120.1030.108 250.2080.2240.216 12.50.3770.3970.387 6.250.7830.7180.751 3.121.1211.0861.104 01.9511.8291.890 | ||||||
货期：3-5 working days
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