首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人Coll2-1酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液样本中的Coll2-1含量。试剂盒检测范围为47 pg/mL-3000 pg/mL，Coll2-1是关节软骨中II型胶原蛋白特异性降解的生物标志物，其水平变化与关节退行性疾病及软骨代谢异常密切相关，在骨关节炎机制研究、药物开发疗效评估及运动医学相关科研领域具有重要应用价值。试剂盒包含预包被抗体、标准品及检测所需试剂，操作流程标准化，适用于体外检测科研样本中Coll2-1的动态变化，可为软骨代谢研究、疾病模型建立及生物标志物筛选提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：Coll2-1
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates
检测范围：47 pg/mL-3000 pg/mL
灵敏度：11.75 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human Coll2-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 85 | ||||
| Range % | 80-90 | |||||
| 1:200 | Average % | 98 | ||||
| Range % | 90-105 | |||||
| 1:400 | Average % | 95 | ||||
| Range % | 90-100 | |||||
| 1:800 | Average % | 92 | ||||
| Range % | 87-97 | |||||
回收率：
| The recovery of human Coll2-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 83-95 | ||||
| EDTA plasma (n=4) | 98 | 92-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 30002.5132.4872.5002.417 15001.8471.7941.8211.738 7501.1981.1121.1551.072 3750.7540.7090.7320.649 187.50.3510.3370.3440.261 940.1980.1830.1910.108 470.1340.1250.1300.047 00.0840.0810.083 | ||||||
货期：3-5 working days
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