首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人胶原凝集素-12(COLEC12)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的COLEC12含量。COLEC12是一种多功能清道夫受体，具有低密度脂蛋白颗粒结合活性、模式识别受体活性和清道夫受体活性，在免疫应答中发挥作用，位于含有胶原的细胞外基质中，参与多种生物学过程。研究机制涉及其在宿主防御、细胞粘附和信号传导中的作用。试剂盒检测范围为0.312 ng/mL-20 ng/mL，科研领域可用于探究COLEC12在感染性疾病、免疫调控机制、慢性炎症或肿瘤微环境中的表达特征，例如研究病原体 - 宿主互作模型中的分子响应、评估组织损伤修复过程中COLEC12的动态变化，或解析特定疾病模型中该蛋白的表达调控网络，为相关基础研究提供可靠的检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CL P1 ELISA Kit; CL-P1 ELISA Kit; CLP1 ELISA Kit; COL12_HUMAN ELISA Kit; COLEC 12 ELISA Kit; colec12 ELISA Kit; Collectin placenta 1 ELISA Kit; Collectin placenta protein 1 ELISA Kit; Collectin sub family member 12 ELISA Kit; Collectin-12 ELISA Kit; hCL P1 ELISA Kit; hCL-P1 ELISA Kit; NSR2 ELISA Kit; Nurse cell scavenger receptor 2 ELISA Kit; SCARA4 ELISA Kit; Scavenger receptor class A member 4 ELISA Kit; Scavenger receptor with C type lectin ELISA Kit; Scavenger receptor with C-type lectin ELISA Kit; SRCL ELISA Kit
缩写：COLEC12
Uniprot No.：Q5KU26
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human COLEC12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 84 | |||||
| Range % | 80-88 | ||||||
| 1:2 | Average % | 85 | |||||
| Range % | 82-89 | ||||||
| 1:4 | Average % | 91 | |||||
| Range % | 86-95 | ||||||
| 1:8 | Average % | 104 | |||||
| Range % | 101-107 | ||||||
回收率：
| The recovery of human COLEC12 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 89 | 85-93 | |||||
| EDTA plasma (n=4) | 90 | 85-95 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected202.5412.6982.6202.498102.0392.0002.0201.89851.3071.3681.3381.2162.50.7170.7380.7280.6061.250.4020.3980.4000.2780.6250.2810.2780.2800.1580.3120.1830.1790.1810.05900.1230.1200.122 | |||||||
货期：3-5 working days
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