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产品描述：人IV形胶原蛋白α3链(COL4A3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的COL4A3含量。COL4A3编码IV型胶原蛋白α3链，其突变可致Alport综合征等疾病。研究机制方面，聚焦其在基底膜结构维持中的作用，探索突变引发疾病的分子机制，也研究其与肿瘤微环境等关联，以寻找新疗法和诊断标志物。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于体外研究COL4A3在肾纤维化进程中的动态变化、评估肾损伤模型的病理进展，或筛选调节基底膜代谢的候选化合物。可为肾脏疾病机制研究和新药开发提供可靠的实验工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Alpha 3 type IV collagen ELISA Kit; Alpha3 type IV collagen ELISA Kit; CO4A3_HUMAN ELISA Kit; COL4A 3 ELISA Kit; Col4a3 ELISA Kit; Collagen alpha 3(IV) chain ELISA Kit; Collagen IV alpha 3 polypeptide ELISA Kit; Collagen type IV alpha 3 (Goodpasture antigen) ELISA Kit; Collagen type IV alpha 3 ELISA Kit; Collagen type IV alpha 3 chain ELISA Kit; Goodpasture antigen ELISA Kit; OTTHUMP00000195044 ELISA Kit; Tumstatin ELISA Kit
缩写：COL4A3
Uniprot No.：Q01955
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human COL4A3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:5 | Average % | 104 | |||||
| Range % | 101-107 | ||||||
| 1:10 | Average % | 91 | |||||
| Range % | 88-98 | ||||||
| 1:20 | Average % | 96 | |||||
| Range % | 91-103 | ||||||
| 1:40 | Average % | 89 | |||||
| Range % | 86-92 | ||||||
回收率：
| The recovery of human COL4A3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 102 | 99-105 | |||||
| EDTA plasma (n=4) | 96 | 92-100 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected 102.5442.5112.5282.347 52.0712.0232.0471.866 2.51.3561.4581.4071.226 1.250.7760.7740.7750.594 0.6250.4500.4880.4690.288 0.3120.2920.2710.2820.101 0.1560.2080.2200.2140.033 00.1780.1840.181 | |||||||
货期：3-5 working days
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