首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人IV型胶原α-2链(COL4A2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的COL4A2含量。COL4A2是重要靶点。它编码IV型胶原蛋白α2链，在维持组织器官结构和功能、细胞黏附等方面起关键作用。研究机制涉及探究其在疾病如肿瘤、肾病等中的表达变化及影响，为开发相关诊断方法和治疗药物提供依据。试剂盒检测范围为47 pg/mL-3000 pg/mL，适用于研究基底膜异常相关疾病机制，如肾小球硬化、肿瘤侵袭转移或血管生成调控等领域。科研人员可借助该产品探索纤维化疾病进程、细胞外基质重塑或遗传性基底膜病变模型中的COL4A2动态变化，为组织微环境研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Canstatin ELISA Kit; CO4A2_HUMAN ELISA Kit; COL4A 2 ELISA Kit; Col4a2 ELISA Kit; Collagen alpha 2(IV) chain ELISA Kit; Collagen; type IV; alpha 2 ELISA Kit
缩写：COL4A2
Uniprot No.：P08572
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：47 pg/mL-3000 pg/mL
灵敏度：11.75 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human COL4A2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:20 | Average % | 92 | |||||
| Range % | 84-95 | ||||||
| 1:40 | Average % | 103 | |||||
| Range % | 96-106 | ||||||
| 1:80 | Average % | 89 | |||||
| Range % | 84-92 | ||||||
| 1:160 | Average % | 106 | |||||
| Range % | 94-109 | ||||||
回收率：
| The recovery of human COL4A2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 101 | 93-104 | |||||
| EDTA plasma (n=4) | 92 | 85-96 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected30002.6612.7612.7112.61115002.1682.2432.2062.1067501.7741.7351.7551.6553751.2891.2471.2681.168187.50.6410.6650.6530.553940.4030.4110.4070.307470.2400.2460.2430.14300.0980.1020.100 | |||||||
货期：3-5 working days
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