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产品描述：猪Ⅲ型胶原(Col Ⅲ)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的Col Ⅲ含量。Col Ⅲ（胶原蛋白Ⅲ）是细胞外基质的主要成分之一，参与维持肺动脉管壁的弹性和完整性。在肺动脉高压（PAH）等疾病中，TGF-β1等因子促进PASMCs外胶原沉积，导致肺血管重构。低浓度紫杉醇（PTX）可通过抑制TGF-β1/Smad3信号通路，降低COL Ⅲ表达，从而逆转纤维化进程。试剂盒检测范围为6.25 ng/mL-400 ng/mL，适用于探究猪模型在纤维化机制、心血管疾病或皮肤再生等领域的科研需求，为组织工程、病理模型构建及分子机制研究提供可靠数据支持，是基础研究与转化医学中胶原代谢相关课题的理想工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Collagen Type 3,Col 3,_x000D_ Collagen Type III,Col III
缩写：Col Ⅲ
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：6.25 ng/mL-400 ng/mL
灵敏度：1.56 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig Col Ⅲ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 91-103 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 97-107 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 85-97 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 89-95 | |||||
回收率：
| The recovery of pig Col Ⅲ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 88-96 | ||||
| EDTA plasma (n=4) | 97 | 92-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 4001.8981.8781.8881.712 2001.2791.2561.2681.092 1000.8340.9000.8670.691 500.6150.6350.6250.449 250.4110.4350.4230.247 12.50.3310.3550.3430.167 6.250.2980.2870.2930.117 00.1750.1770.176 | ||||||
货期：3-5 working days
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