首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人Ⅲ型胶原(Col Ⅲ)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的Col Ⅲ含量。人Ⅲ型胶原（Col Ⅲ）是人体皮肤中重要的胶原蛋白类型，主要存在于婴儿皮肤和血管内膜。其研究背景涉及胶原蛋白的生物学功能，如支撑皮肤结构、促进细胞迁移和修复。研究机制则聚焦于其三螺旋结构、细胞粘附性及其在皮肤修复和抗衰老中的作用。试剂盒检测范围为12.5 ng/mL-800 ng/mL，适用于体外研究Col Ⅲ在皮肤老化、肝纤维化、心血管疾病或组织工程修复等领域的表达调控，可为探索纤维化疾病分子机制或评估抗纤维化药物效果提供数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Collagen Type 3,Col 3,_x000D_ Collagen Type III,Col III
缩写：Col Ⅲ
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：12.5 ng/mL-800 ng/mL
灵敏度：3.12 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human Col Ⅲ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 86-96 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 97-105 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 85-98 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 91-102 | |||||
回收率：
| The recovery of human Col Ⅲ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 86 | 80-94 | ||||
| EDTA plasma (n=4) | 97 | 92-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 8002.8502.9632.9072.778 4002.2552.2032.2292.100 2001.2791.2561.2681.139 1000.6590.6550.6570.528 500.4380.4750.4570.328 250.2560.2420.2490.120 12.50.1840.1720.1780.049 00.1260.1320.129 | ||||||
货期：3-5 working days
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