首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠Ⅱ型胶原(Col Ⅱ)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的Col Ⅱ含量。Col Ⅱ即Ⅱ型胶原，是软骨基质的主要成分。其与关节炎等疾病密切相关，自身抗体识别可触发免疫反应。研究机制主要围绕其在软骨稳态维持、免疫调节中的作用，探索其在疾病诊断、治疗靶点方面的价值。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，该产品可广泛应用于软骨发育、退行性关节疾病机制研究、药物或生物材料对软骨代谢影响的体外评估等科研领域，为探索Col Ⅱ在骨骼系统相关疾病中的作用提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Collagen Type 2,Col 2,_x000D_ Collagen Type II,Col II
缩写：Col Ⅱ
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat Col Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 87 | ||||
| Range % | 83-92 | |||||
| 1:200 | Average % | 94 | ||||
| Range % | 90-99 | |||||
| 1:400 | Average % | 92 | ||||
| Range % | 88-96 | |||||
| 1:800 | Average % | 102 | ||||
| Range % | 98-105 | |||||
回收率：
| The recovery of rat Col Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 93-96 | ||||
| EDTA plasma (n=4) | 98 | 89-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 10002.5842.5992.5922.438 5002.2172.2532.2352.081 2501.7651.7921.7791.625 1251.2921.2641.2781.124 62.50.7890.7730.7810.627 31.20.3960.3880.3920.238 15.60.2570.2690.2630.109 00.1530.1550.154 | ||||||
货期：3-5 working days
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