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产品描述：人肥大细胞类胰凝乳蛋白酶酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、组织培养上清液、组织匀浆样本中的CMA1含量。CMA1，即糜酶/CMA1蛋白，是肥大细胞分泌的一种蛋白酶，参与血管活性肽生成、细胞外基质降解和腺体分泌调节。CMA1的研究涉及其在哮喘、动脉粥样硬化等疾病中的作用机制。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于肥大细胞活化机制研究、炎症相关疾病模型构建、药物干预效果评估等科研场景，为免疫学、病理学及药理学领域提供可靠的数据支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Alpha-chymase ELISA Kit; Chymase 1 ELISA Kit; Chymase 1 mast cell ELISA Kit; chymase 1 preproprotein transcript E ELISA Kit; chymase 1 preproprotein transcript I ELISA Kit; Chymase ELISA Kit; Chymase; heart ELISA Kit; Chymase; mast cell ELISA Kit; CMA1 ELISA Kit; CMA1_HUMAN ELISA Kit; CYH ELISA Kit; CYM ELISA Kit; EC 3.4.21.39 ELISA Kit; Mast cell chymase 1 ELISA Kit; Mast cell protease 3 ELISA Kit; Mast cell protease 5 ELISA Kit; Mast cell protease I ELISA Kit; Mast cell protease III ELISA Kit; Mcp-5 ELISA Kit; MCP3P ELISA Kit; Mcpt5 ELISA Kit; MCT1 ELISA Kit; MGC119890 ELISA Kit; MGC119891 ELISA Kit; MMCP-5 ELISA Kit
缩写：CMA1
Uniprot No.：P23946
种属：Homo sapiens (Human)
样本类型：serum, cell culture supernates, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human mast cell chymase (CMA1) in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 98 | ||||
| Range % | 94-103 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 84-95 | |||||
| 1:4 | Average % | 99 | ||||
| Range % | 94-104 | |||||
| 1:8 | Average % | 89 | ||||
| Range % | 85-94 | |||||
回收率：
| The recovery of human mast cell chymase(CMA1) spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 85-95 | ||||
| EDTA plasma (n=4) | 101 | 95-107 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.7352.7892.7622.65652.3972.3852.3912.2852.51.8321.8691.8511.7451.251.2561.2411.2491.1430.6250.7250.7560.7410.6350.3120.4530.4680.4610.3550.1560.2890.2810.2850.17900.1050.1070.106 | ||||||
本试剂盒所含材料：
A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human mast cell Chymase antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled mast cell Chymase antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibodyDiluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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