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产品描述:小鼠C型凝集素结构域家族9成员A(CLEC9A)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆、细胞裂解物样本中的CLEC9A含量。CLEC9A是一个具有研究价值的靶点。它在免疫系统中发挥作用,能识别受损细胞释放的特定分子模式。相关研究致力于揭示其信号传导机制,探索它在免疫激活、抗肿瘤免疫等方面的潜力,有望为开发免疫治疗新策略提供方向。试剂盒检测范围为0.312 ng/mL-20 ng/mL,适用于科研场景下探究CLEC9A在免疫调控、炎症反应或病原体感染中的分子机制,亦可作为体外实验中的生物标志物检测工具,支持通过多种生物样本(如血清中的可溶性蛋白或组织微环境中的表达水平)进行定量分析,为免疫学、细胞生物学相关研究提供标准化检测方案。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:C type lectin domain family 9; member A ELISA Kit; C-type lectin domain family 9 member A ELISA Kit; CLC9A_HUMAN ELISA Kit; CLEC9A ELISA Kit; DNGR1 ELISA Kit; HEEE9341 ELISA Kit; PRO34046 ELISA Kit; UNQ9341 ELISA Kit
缩写:CLEC9A
Uniprot No.:Q6UXN8
种属:Homo sapiens (Human)
样本类型:serum, plasma, tissue homogenates, cell lysates
检测范围:0.312 ng/mL-20 ng/mL
灵敏度:0.078 ng/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Immunology
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
Inter-assay Precision (Precision between assays): CV%<10% | |||||||
Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human CLEC9A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
Sample | Serum(n=4) | ||||||
1:1 | Average % | 89 | |||||
Range % | 85-93 | ||||||
1:2 | Average % | 104 | |||||
Range % | 100-108 | ||||||
1:4 | Average % | 89 | |||||
Range % | 85-93 | ||||||
1:8 | Average % | 108 | |||||
Range % | 104-112 |
回收率:
The recovery of human CLEC9A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
Sample Type | Average % Recovery | Range | |||||
Serum (n=5) | 88 | 84-93 | |||||
EDTA plasma (n=4) | 106 | 103-109 | |||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
ng/mlOD1OD2AverageCorrected 202.2732.1092.1912.038 101.5751.6041.5901.437 51.1791.2051.1921.039 2.50.8250.8250.8250.672 1.250.5250.5320.5290.376 0.6250.3750.3680.3720.219 0.3120.2640.2610.2630.110 00.1540.1520.153 |
货期:3-5 working days
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