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产品描述：人C型凝集素域7家族成员A(CLEC7A)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CLEC7A含量。CLEC7A 是重要的免疫相关靶点。它在天然免疫应答中起关键作用，能识别真菌细胞壁上的β-葡聚糖。相关研究围绕其识别配体机制、信号转导途径展开，旨在深入了解免疫激活过程，为开发针对 CLEC7A 的免疫治疗药物提供理论支撑。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，本试剂盒适用于体外定量分析不同样本中CLEC7A的蛋白表达水平，可为感染性疾病机制、自身免疫病理研究或免疫调节药物开发等科研领域提供可靠数据支持，尤其适合探究CLEC7A在免疫识别、细胞信号转导及相关疾病模型中的功能变化。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Beta GR ELISA Kit; Beta-glucan receptor ELISA Kit; BGR ELISA Kit; C type calcium dependent carbohydrate recognition domain lectin superfamily member 12 ELISA Kit; C type lectin domain family 7 member a ELISA Kit; C-type lectin domain family 7 member A ELISA Kit; C-type lectin superfamily member 12 ELISA Kit; CANDF4 ELISA Kit; CLC7A_HUMAN ELISA Kit; CLEC7A ELISA Kit; CLEC7A protein ELISA Kit; Clecsf12 ELISA Kit; DC-associated C-type lectin 1 ELISA Kit; Dectin 1 ELISA Kit; DECTIN 1 receptor ELISA Kit; Dectin-1 ELISA Kit; Dectin1 ELISA Kit; Dendritic cell associated C type lectin 1 ELISA Kit; Dendritic cell-associated C-type lectin 1 ELISA Kit; Lectin like receptor 1 ELISA Kit; Putative transmembrane protein dectin 1 ELISA Kit
缩写：CLEC7A
Uniprot No.：Q9BXN2
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CLEC7A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||||||||
| Sample | Serum(n=4) | ||||||||||||
| 1:1 | Average % | 87 | |||||||||||
| Range % | 84-95 | ||||||||||||
| 1:2 | Average % | 90 | |||||||||||
| Range % | 85-95 | ||||||||||||
| 1:4 | Average % | 97 | |||||||||||
| Range % | 92-101 | ||||||||||||
| 1:8 | Average % | 93 | |||||||||||
| Range % | 89-99 | ||||||||||||
回收率：
| The recovery of human CLEC7A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||||||||
| Sample Type | Average % Recovery | Range | |||||||||||
| Serum (n=5) | 100 | 96-104 | |||||||||||
| EDTA plasma (n=4) | 96 | 90-102 | |||||||||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||
| pg/mlOD1OD2AverageCorrected20002.2462.2972.2722.15410001.8211.8621.8421.7245001.3151.3361.3261.2082500.8870.8740.8810.7631250.4530.4630.4580.34062.50.3220.3420.3320.21431.250.2620.2540.2580.14000.1190.1170.118 | |||||||||||||
货期：3-5 working days
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