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产品描述：人C型凝集素结构域家族2成员D(CLEC2D)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的CLEC2D含量。CLEC2D作为靶点，在人体生理疾病过程中可能有重要作用。目前相关研究或聚焦于其在免疫调节、细胞信号传导等机制方面。深入探究其背景及作用机制，能为开发针对性治疗方法、药物等提供理论支撑，助力攻克相关疾病难题。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于探索CLEC2D在免疫调节、肿瘤微环境、感染性疾病或自身免疫性疾病中的分子机制研究，为体外实验提供可靠的定量数据支持。适用于基础科研中细胞模型、动物模型或临床前样本的分析，为研究CLEC2D的功能及其相关通路提供高效工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：C type lectin related f ELISA Kit; C type lectin superfamily 2; member D ELISA Kit; C-type lectin domain family 2 member D ELISA Kit; CLAX ELISA Kit; CLC2D_HUMAN ELISA Kit; CLEC2D ELISA Kit; CLEC2D protein ELISA Kit; Clec2d5 ELISA Kit; L LT1 ELISA Kit; Lectin like NK cell receptor ELISA Kit; Lectin like transcript 1 ELISA Kit; Lectin-like NK cell receptor ELISA Kit; Lectin-like transcript 1 ELISA Kit; LLT-1 ELISA Kit; LLT1 ELISA Kit; OCIL ELISA Kit; Osteoclast inhibitory lectin ELISA Kit
缩写：CLEC2D
Uniprot No.：Q9UHP7
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CLEC2D in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 85-93 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 92-99 | |||||
| 1:4 | Average % | 87 | ||||
| Range % | 83-91 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 84-97 | |||||
回收率：
| The recovery of human CLEC2D spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 87-97 | ||||
| EDTA plasma (n=4) | 96 | 90-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.5752.6002.5882.44510002.2152.1472.1812.0385001.5331.4981.5161.3732500.9010.9440.9230.7801250.5310.5650.5480.40562.50.3650.3440.3550.21231.250.2580.2500.2540.11100.1390.1470.143 | ||||||
货期：3-5 working days
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