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产品描述：人C型凝集素结构域家族18成员A(CLEC18A)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CLEC18A含量。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于基础科研领域中对CLEC18A表达调控机制的研究，例如通过血清或血浆样本评估其在系统免疫中的动态变化，或利用组织匀浆分析特定器官中该蛋白的分布特征。实验操作兼容常规实验室条件，可为免疫学、细胞信号通路及疾病模型研究提供可靠的数据支持，助力探索CLEC18A在生理病理过程中的分子作用。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：C-type lectin domain family 18 member A ELISA Kit; CL18A_HUMAN ELISA Kit; CLEC18A ELISA Kit; FLJ77955 ELISA Kit; Mannose receptor-like protein 2 ELISA Kit; MGC164804 ELISA Kit; Mrcl ELISA Kit; MRCL1 ELISA Kit; MRLP2 ELISA Kit
缩写：CLEC18A
Uniprot No.：A5D8T8
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CLEC18A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 88-97 | |||||
| 1:2 | Average % | 99 | ||||
| Range % | 95-107 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 85-93 | |||||
| 1:8 | Average % | 84 | ||||
| Range % | 80-88 | |||||
回收率：
| The recovery of human CLEC18A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 100 | 95-107 | ||||
| EDTA plasma (n=4) | 90 | 85-94 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 202.4392.5502.4952.362 101.8251.9561.8911.758 51.2731.2241.2491.116 2.50.6710.6930.6820.549 1.250.4570.4270.4420.309 0.6250.3090.3170.3130.180 0.3120.2420.2390.2410.108 00.1300.1350.133 | ||||||
货期：3-5 working days
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