首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人封闭蛋白7(CLDN7)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的CLDN7含量。CLDN7是一种紧密连接蛋白，在维持细胞极性和屏障功能方面发挥关键作用。研究发现其在多种癌症中表达异常，与肿瘤的增殖、侵袭和转移等密切相关。深入研究其作用机制，有望为癌症等疾病的诊断和治疗提供新的靶点和思路。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于研究CLDN7在组织屏障调控、肿瘤微环境建立或药物作用机制等领域的生物学功能；可兼容细胞实验、动物模型及临床前样本分析，帮助科研人员高效完成跨物种或跨模型的蛋白表达研究本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CLDN7; CEPTRL2; CPETRL2; Claudin-7; CLDN-7
缩写：CLDN7
Uniprot No.：O95471
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CLDN7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 96 | |||||
| Range % | 91-102 | ||||||
| 1:2 | Average % | 107 | |||||
| Range % | 103-112 | ||||||
| 1:4 | Average % | 92 | |||||
| Range % | 85-96 | ||||||
| 1:8 | Average % | 87 | |||||
| Range % | 83-92 | ||||||
回收率：
| The recovery of human CLDN7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 89 | 84-93 | |||||
| EDTA plasma (n=4) | 104 | 97-108 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected10002.4122.3752.3942.2615001.7241.6031.6641.5312501.0871.0521.0700.9371250.6650.6580.6620.52962.50.4620.4530.4580.32531.20.3460.3230.3350.20215.60.2310.2270.2290.09600.1340.1320.133 | |||||||
货期：3-5 working days
×
