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产品描述：人细胞连接相关蛋白2(CLASP2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的CLASP2含量。CLASP2作为靶点，在细胞生命活动中扮演重要角色。其它与细胞微管的稳定和动态调节密切相关。研究机制上，主要聚焦于其如何调控微管动力学，以及在细胞分裂、迁移等过程中发挥的作用，对了解细胞生理及相关疾病机制有重要意义。试剂盒检测范围为23.5 pg/mL-1500 pg/mL，适用于探究CLASP2在神经生物学、细胞迁移机制、癌症侵袭转移等领域的表达调控，以及相关疾病模型的分子机制研究，为体外实验提供高灵敏度的定量检测方案。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CLASP2; KIAA0627; CLIP-associating protein 2; Cytoplasmic linker-associated protein 2; Protein Orbit homolog 2; hOrbit2
缩写：CLASP2
Uniprot No.：O75122
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：23.5 pg/mL-1500 pg/mL
灵敏度：5.86 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CLASP2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 87-96 | |||||
| 1:2 | Average % | 104 | ||||
| Range % | 100-110 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 85-93 | |||||
| 1:8 | Average % | 86 | ||||
| Range % | 81-90 | |||||
回收率：
| The recovery of human CLASP2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 88-97 | ||||
| EDTA plasma (n=4) | 96 | 90-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected15002.7312.6122.6722.5017502.2072.1292.1681.9973751.4951.4081.4521.281187.50.8040.8550.8300.659940.5470.5140.5310.360470.3290.3380.3340.16323.50.2360.2290.2330.06200.1730.1680.171 | ||||||
货期：3-5 working days
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