首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠肌酸激酶同工酶MM(CK-MM)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CK-MM含量。CK-MM即肌酸激酶同工酶，主要存在于骨骼肌中。它可作为诊断急性心肌梗死等疾病的重要指标，反映心肌和骨骼肌损伤情况。研究机制方面，通过检测血液中CK-MM活性变化，能辅助判断病情严重程度与预后，为临床治疗提供关键依据。试剂盒检测范围为7.8 μU/mL-500 μU/mL，适用于运动医学、肌肉疾病模型构建、药物毒理评估等科研场景中CK - MM的动态监测。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：CK-MM
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：7.8 μU/mL-500 μU/mL
灵敏度：1.95 μU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat CK-MM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 105 | ||||
| Range % | 101-109 | |||||
| 1:200 | Average % | 87 | ||||
| Range % | 84-90 | |||||
| 1:400 | Average % | 102 | ||||
| Range % | 98-106 | |||||
| 1:800 | Average % | 105 | ||||
| Range % | 101-109 | |||||
回收率：
| The recovery of rat CK-MM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-98 | ||||
| EDTA plasma (n=4) | 87 | 85-90 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| μU/mlOD1OD2Average5000.0800.0780.0792500.1290.1310.1301250.2010.2150.20862.50.3210.3480.33531.250.5420.5010.52215.60.8380.8680.8537.81.1681.1981.18302.0251.9872.006 | ||||||
货期：3-5 working days
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