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产品描述：人肌酸激酶同工酶MM(CK-MM)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CK-MM含量。CK-MM是一种主要存在于骨骼肌和心脏中的肌酸激酶同工酶，参与能量代谢和肌肉收缩。研究显示，CK-MM在肌肉损伤和心肌梗死后水平升高，可用于相关疾病的诊断。其研究机制涉及在肌肉损伤或心肌梗死后，CK-MM从细胞中释放到血液中，通过检测血液中的CK-MM水平来诊断疾病。试剂盒检测范围为3.12 mU/mL-200 mU/mL，可应用于运动医学研究、肌肉损伤机制探索、药物干预效果评估等科研领域，为肌肉代谢相关疾病的分子机制研究提供稳定可靠的检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：CK-MM
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 mU/mL-200 mU/mL
灵敏度：0.78 mU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CK-MM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 81-93 | |||||
| 1:2 | Average % | 111 | ||||
| Range % | 99-114 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 83-94 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 84-96 | |||||
回收率：
| The recovery of human CK-MM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 83-95 | ||||
| EDTA plasma (n=4) | 104 | 91-107 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| mU/mlOD1OD2AverageCorrected 2002.1232.0882.1061.969 1001.6651.6341.6501.513 501.1281.1781.1531.016 250.6600.6420.6510.514 12.50.3820.3970.3900.253 6.250.2510.2440.2480.111 3.120.1650.1730.1690.032 00.1360.1380.137 | ||||||
货期：3-5 working days
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