首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠肌酸激酶同工酶MB(CK-MB)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、尿液、组织匀浆、血浆样本中的CK-MB含量。CK-MB即肌酸激酶同工酶，主要存在于心肌中。测定其在血液中的含量，能反映心肌损伤情况，是诊断急性心肌梗死的重要指标。研究机制主要是在心肌受损时，CK-MB释放入血，通过检测血中浓度变化辅助病情判断。试剂盒检测范围为0.312 ng/mL-20 ng/mL，广泛应用于心血管疾病动物模型研究，如心肌损伤机制探索、心脏保护药物筛选等科研领域。适用于心肌损伤动态监测、药物干预效果评估等实验需求。为心血管相关基础研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：CK-MB
种属：Rattus norvegicus (Rat)
样本类型：serum, urine, tissue homogenates, plasma
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat CK-MB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 100 | ||||
| Range % | 98-103 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 92-100 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 87-98 | |||||
| 1:8 | Average % | 89 | ||||
| Range % | 84-94 | |||||
回收率：
| The recovery of rat CK-MB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 100 | 96-104 | ||||
| EDTA plasma (n=4) | 93 | 89-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 202.6462.6872.6672.564 101.8341.8561.8451.742 51.0961.0991.0980.995 2.50.5930.5830.5880.485 1.250.3560.3680.3620.259 0.6250.2290.2490.2390.136 0.3120.1650.1740.1700.067 00.1010.1050.103 | ||||||
本试剂盒所含材料：
A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-Rat CK-MB antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled CK-MB antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
×
