首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人腱蛋白样蛋白1(CHRDL1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CHRDL1含量。人腱蛋白样蛋白1（CHRDL1）是一种与BMP4结合的拮抗剂，能阻断其受体相互作用，将神经干细胞从胶质生成重定向到神经发生，支持大脑中的神经元分化。研究显示，CHRDL1在背腹轴形成、胚胎骨发育和视网膜血管生成中发挥重要作用，并通过调节BMP4的作用来调节视网膜血管生成。试剂盒检测范围为0.156 ng/mL-10 ng/mL，支持科研人员开展CHRDL1在神经生物学、骨骼发育、肿瘤微环境调控等领域的机制研究，尤其适用于探究CHRDL1在疾病模型中的动态表达及其与BMP通路互作的分子机制。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CHL ELISA Kit; chordin like 1 ELISA Kit; Chordin-like protein 1 ELISA Kit; CHRDL1 ELISA Kit; CRDL1_HUMAN ELISA Kit; dA141H5.1 ELISA Kit; neuralin 1 ELISA Kit; Neuralin-1 ELISA Kit; Neurogenesin-1 ELISA Kit; NRLN1 ELISA Kit; Ventroptin ELISA Kit; VOPT ELISA Kit
缩写：CHRDL1
Uniprot No.：Q9BU40
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CHRDL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 84-93 | |||||
| 1:2 | Average % | 104 | ||||
| Range % | 98-107 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 88-99 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 91-99 | |||||
回收率：
| The recovery of human CHRDL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 84-94 | ||||
| EDTA plasma (n=4) | 99 | 94-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.5512.4872.5192.35452.1642.1122.1381.9732.51.6511.6881.6701.5051.251.0431.0321.0380.8730.6250.6600.6450.6530.4880.3120.4740.4670.4710.3060.1560.2680.2720.2700.10500.1630.1670.165 | ||||||
货期：3-5 working days
×
