首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人几丁质酶1(壳三糖酶)(CHIT1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的CHIT1含量。CHIT1是一种重要靶点。它在人体生理及病理过程中有着关键作用。研究发现其可能与多种疾病的发生发展相关，如炎症性疾病等。机制方面，多聚焦于其在细胞信号传导、炎症因子调控等环节影响疾病进程，为相关药物研发提供了方向。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于体外培养细胞分泌研究、动物模型组织分析或临床样本中CHIT1动态变化的定量评估；广泛应用于免疫调控机制研究、感染性疾病模型建立、药物干预效果评估等科研领域，为探索CHIT1在代谢异常或炎性微环境中的功能提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CHI3 ELISA Kit; CHIT ELISA Kit; CHIT1 ELISA Kit; CHIT1_HUMAN ELISA Kit; CHITD ELISA Kit; Chitinase 1 ELISA Kit; Chitinase 1 (chitotriosidase) ELISA Kit; Chitinase-1 ELISA Kit; Chitotriosidase ELISA Kit; Chitotriosidase-1 ELISA Kit; EC 3.2.1.14 ELISA Kit; FLJ00314 ELISA Kit; MGC125322 ELISA Kit; Plasma methylumbelliferyl tetra N acetylchitotetraoside hydrolase ELISA Kit
缩写：CHIT1
Uniprot No.：Q13231
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CHIT1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 86 | ||||
| Range % | 80-96 | |||||
| 1:200 | Average % | 94 | ||||
| Range % | 91-98 | |||||
| 1:400 | Average % | 87 | ||||
| Range % | 80-101 | |||||
| 1:800 | Average % | 100 | ||||
| Range % | 86-105 | |||||
回收率：
| The recovery of human CHIT1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 80-98 | ||||
| EDTA plasma (n=4) | 96 | 90-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.7542.6212.6882.58010002.3242.2022.2632.1555001.5431.4971.5201.4122500.9070.8450.8760.7681250.4460.4210.4340.32662.50.2360.2210.2290.12131.250.1390.1370.1380.03000.1090.1070.108 | ||||||
货期：3-5 working days
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