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产品描述:人补体因子H相关蛋白1(CFHR1)酶联免疫试剂盒为竞争法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的CFHR1含量。CFHR1是一个研究靶点。它可能参与补体系统的调控等生理过程,在维持机体免疫平衡等方面有重要意义。目前研究聚焦于其在疾病发生发展机制中的作用,如与肾脏疾病等相关性,探索其在疾病诊断、治疗和发病机制研究的价值。试剂盒检测范围为25 ng/mL-1600 ng/mL,该产品适用于科研领域的基础研究,例如探索CFHR1在补体系统相关疾病中的表达水平变化、评估其在炎症或免疫调节中的分子机制,或用于生物样本中CFHR1浓度的定量分析,为疾病机制研究提供实验支持。试剂盒严格匹配标准品浓度梯度,实验结果稳定可靠,适用于实验室常规检测需求。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:CFHL ELISA Kit; CFHL1 ELISA Kit; CFHL1P ELISA Kit; CFHR1 ELISA Kit; CFHR1P ELISA Kit; Complement factor H related 1 ELISA Kit; Complement factor H related 1 pseudogene ELISA Kit; Complement factor H related protein 1 precursor ELISA Kit; Complement factor H-related protein 1 ELISA Kit; FHR 1 ELISA Kit; FHR-1 ELISA Kit; FHR1 ELISA Kit; FHR1_HUMAN ELISA Kit; H factor (complement) like 1 ELISA Kit; H factor (complement) like 2 ELISA Kit; H factor like protein 1 ELISA Kit; H factor-like protein 1 ELISA Kit; H-factor-like 1 ELISA Kit; H36 1 ELISA Kit; H36 2 ELISA Kit; H36 ELISA Kit; HFL1 ELISA Kit; HFL2 ELISA Kit; MGC104329 ELISA Kit
缩写:CFHR1
Uniprot No.:Q03591
种属:Homo sapiens (Human)
样本类型:serum, plasma, tissue homogenates
检测范围:25 ng/mL-1600 ng/mL
灵敏度:12.5 ng/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Immunology
测定原理:quantitative
测定方法:Competitive
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human CFHR1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:100 | Average % | 98 | ||||
Range % | 92-106 | |||||
1:200 | Average % | 95 | ||||
Range % | 91-99 | |||||
1:400 | Average % | 102 | ||||
Range % | 98-106 | |||||
1:800 | Average % | 85 | ||||
Range % | 80-89 |
回收率:
The recovery of human CFHR1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 92 | 88-98 | ||||
EDTA plasma (n=4) | 102 | 98-108 | ||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
ng/mlOD1OD2Average 16000.0890.0900.090 8000.1640.1670.166 4000.2790.2880.284 2000.4910.5020.497 1000.7540.7400.747 501.0681.0591.064 251.5451.4471.496 02.5212.6232.572 |
货期:3-5 working days
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