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产品描述：人腺苷脱氨酶CECR1(CECR1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CECR1含量。CECR1即胞外5'-核苷酸酶相关蛋白1。它在免疫调节等生理过程中发挥作用。研究机制方面，其与多种细胞功能及信号通路存在关联，可能参与炎症反应调控等，目前对它在疾病发生发展机制及潜在治疗靶点作用的研究正逐步深入。试剂盒检测范围为0.312 ng/mL-20 ng/mL，该产品支持科研人员探究CECR1在心血管疾病、炎症性疾病模型中的调控机制，或用于细胞因子网络研究、药物干预效果评估等基础研究领域，尤其适合处理微量样本的实验设计。试剂盒经严格验证，适用于人源样本检测，操作简便且重复性良好，为实验室开展CECR1相关分子机制研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ADA2 ELISA kit; ADGF ELISA kit; CECR1 ELISA kit; IDGFLAdenosine deaminase 2 ELISA kit; EC 3.5.4.4 ELISA kit; Cat eye syndrome critical region protein 1 ELISA kit
缩写：CECR1
Uniprot No.：Q9NZK5
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CECR1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 105 | |||||
| Range % | 101-109 | ||||||
| 1:2 | Average % | 92 | |||||
| Range % | 88-96 | ||||||
| 1:4 | Average % | 89 | |||||
| Range % | 84-93 | ||||||
| 1:8 | Average % | 96 | |||||
| Range % | 91-100 | ||||||
回收率：
| The recovery of human CECR1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 87 | 82-91 | |||||
| EDTA plasma (n=4) | 93 | 89-97 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected202.4632.5182.4912.307101.9541.8941.9241.74051.1151.1601.1380.9542.50.7160.6790.6980.5141.250.4010.4270.4140.2300.6250.2790.2910.2850.1010.3120.2270.2350.2310.04700.1820.1860.184 | |||||||
货期：3-5 working days
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